Validation of PON1 enzyme activity assays for longitudinal studies
نویسندگان
چکیده
منابع مشابه
Validation of PON1 enzyme activity assays for longitudinal studies.
BACKGROUND Paraoxonase (PON1) enzymatic activity assays are used to characterize sensitivity to organophosphates and oxidative stress. Length of sample storage, temperature and other factors may influence variability of PON1 measurements, especially in longitudinal studies. METHODS Effects of assay temperature, storage duration up to 7 y (-80 degrees C), freeze-thaw cycles, the type of specim...
متن کاملDevelopmental Changes in PON1 Enzyme Activity in Young Children and Effects of PON1 Polymorphisms
BACKGROUND Paraoxonase 1 (PON1) is an enzyme that detoxifies activated organophosphorus pesticides (OPs) and is also involved in oxidative stress pathways. OBJECTIVES PON1 activity in newborns is lower than in adults, but the ontogeny of PON1 activity is poorly characterized in young children. We examined the effects of age and PON1 genotype on enzyme activity in a birth cohort of Mexican-Ame...
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ژورنال
عنوان ژورنال: Clinica Chimica Acta
سال: 2009
ISSN: 0009-8981
DOI: 10.1016/j.cca.2008.12.019